Browsing by Author "Santhy, K S"

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    Anti-proliferative activity of green tea extract in Human Cervical Cancer Cells (HeLa)
    (2013) Santhy, K S
    In this study mitiproliferative effect of Green tea extract was evaluated on HeLa cell line. HeLa cells were cultured in DMEM medium and incubated witli different concentrations (18.75, 37.5, 75, 150 and 300 gg/ml) of methanol extract of Green tea. Cell viability was assessed by MTT assay. Green tea decreased cell viability in malignant cells in a concentration dependent manner. The IC50 value of green tea extract was found to be 111.9pg/inl. It may be concluded that green tea could cause cell death in HeLa cells and can be considered as a promising antiproliferative agent against cervical carcinogenesis. Introduction Cancer
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    Anticancer Activity of Methanol Extract of Green Tea against Cervical Cancer
    (2016) Santhy, K S; Geetha, B
    Identification and development of natural products used for cancer prevention have attracted a lot of attention globally. Herbal extracts with their proven potential and less side effects in therapeutics have replaced the synthetically derived drugs in modern allopathic medication system. In a low resource country like India, an inexpensive dietary chemopreventive intervention would be an attractive adjunct to existing cervical cancer prevention programs. It is observed that the studies on the relationship between cervical cancer and green I tea are limited, and the observational data are very rare. Hence, in this study an attempt was made to find out the efficacy of methanol extract of green tea against cervical cancer cells (HeLa) by in vitro model systems; and to study the DNA repair capacity of green tea extracts on cultured lymphocytes of cervical cancer patients. It was found that the green tea decreased k cell viability in malignant cells in a concentration dependent manner and the IC50 value of green tea extract was found to be 111.9 ygfml. Chromosomal aberration studies indicate that methanolic extract of green tea showed a reduction in the number of Chromatid Type Aberration i (CTA) in the experimental samples when compared to control samples. Hence, it may be concluded that since green tea could cause cell death in HeLa cells and reduce the DNA damage in cultured lymphocytes of cervical cancer patients it can be considered as a potent anticancer agent against cervical carcinogenesis.
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    Antigenotoxic Potential of Punica granatum in Breast Cancer Patients
    (2016) Santhy, K S; Srividya, M
    Cancer is a disease that knows no geographic boundaries. Despite decades of basic and finical research and trials of promising new therapies, cancer remains a major cause of jrbidity and mortality. The post genomic era has now opened new avenues in cancer treatment, vhich is contemplated to be more effective and specific for tumor cells using various methods uailablefor the assessment ofDNA damage and repair in cancer patients such as the bacterial \mes test, the scoring of chromosome aberration (CA), micronuclei and sister chromatid Exchange (SCE) in proliferating cell populations although the frequency of cells with structural iromosomal aberrations in peripheral blood lymphocytes is the first biomarker for rotoxicity that shows an association with overall cancer risk. The present investigation icompasses Single Cell Gel Electrophoresis (SCGE)/Comet assay as an alternative for {togenetic test to assess the extent ofDNA damage in breast cancer patients undergoing ferent modalities of treatment viz., chemotherapy, chemotherapy + surgery, chemotherapy Ifadiotherapy and chemotherapy + surgery + radiotherapy. The present study also comprises • reduction in chromosomal damage by the addition of pomegranate extracts to peripheral ood lymphocyte culture as a biomarker tool for DNA repair capacity of test substance. ?refore, the DNA repair capacity of Pomegranate Fruit Extract (PEE) in cultures of human ripheral blood lymphocytes of breast cancer patients was examined.
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    Antigenotoxic Potential of Punica granatum in Breast Cancer Patients
    (2016) Santhy, K S; Srividya, M
    Cancer is a disease that knows no geographic boundaries. Despite decades of basic and finical research and trials of promising new therapies, cancer remains a major cause of jrbidity and mortality. The post genomic era has now opened new avenues in cancer treatment, vhich is contemplated to be more effective and specific for tumor cells using various methods uailablefor the assessment ofDNA damage and repair in cancer patients such as the bacterial \mes test, the scoring of chromosome aberration (CA), micronuclei and sister chromatid Exchange (SCE) in proliferating cell populations although the frequency of cells with structural iromosomal aberrations in peripheral blood lymphocytes is the first biomarker for rotoxicity that shows an association with overall cancer risk. The present investigation icompasses Single Cell Gel Electrophoresis (SCGE)/Comet assay as an alternative for {togenetic test to assess the extent ofDNA damage in breast cancer patients undergoing ferent modalities of treatment viz., chemotherapy, chemotherapy + surgery, chemotherapy Ifadiotherapy and chemotherapy + surgery + radiotherapy. The present study also comprises • reduction in chromosomal damage by the addition of pomegranate extracts to peripheral ood lymphocyte culture as a biomarker tool for DNA repair capacity of test substance. ?refore, the DNA repair capacity of Pomegranate Fruit Extract (PEE) in cultures of human ripheral blood lymphocytes of breast cancer patients was examined.
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    ANTIMUTAGENIC EFFECT OF GREEN TEA EXTRACTS IN REVERSE MUTATION ASSAY
    (2013) Santhy, K S
    In the present investigation, antimutagenic effect of petroleum ether, chloroform, methanol and water extracts of green tea was evaluated in Salmonella typhimurium TA-98 and TA-100 strains. Well known mutagens like sodium azide and daunomycin were added at a concentration of lOpI and 6pi per plate respectively resulted in the induction of histidine revertant colonies. However addition of 10 pi of petroleum ether, chloroform, ethanol and water extracts of green tea to 10 pi of sodium azide and 6 pi of daunomycin treated plates resulted in the inhibition in the number of histidine revertant colonies. Furthermore, supplementation with all the four extracts of green tea at a concentration of 10 pi per plate respectively in the presence of S9 fraction also led to significant inhibition in sodium azide and daunomycin induced colony formation. The antimutagenic activity of ethanolic extract of green tea was found to be higher than that of the other extracts. Hence the study revealed that green tea has protective efficacy in sodium azide and daunomycin induced mutagenicity in the test microbial system.
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    ANTINEOPLASTIC AND ANTIOXIDANT ACTIVITIES 0¥ ACORUS CALAMUS L ON SWISS ALBINO MICE BEARING DALTON’S ASCITES LYMPHOMA
    (2015) Santhy, K S
    Objective; Ethnohotanical alternates for synthetic allopathic drugs are soon gaining importance in the treatment of diseases due to their fewer side effects. Acorus calamus has been widely used globally due its richness in antioxidant phytoconstituenLs in the treatments yielding beneficial functions. The present study details the in vivo anticancerous effect of A. co/omu.s against Dalton's ascites lymphoma (DALj. Methods; The extract obtained by soxblet extraction was screened for antioxidants by the enzymatic and non-enzymatic methods to indicate any inhibitory properties on DAL induced cells. Results; Soxhiet extraction using organic solvents and water revealed metbanol to yield a maximum percentage of tbe crude extract that was stored for antioxidant and antineoplastic analysis. DAL induced tumor cells in mice revealed decreased levels of superoxide dismutase, catalase, glutathione peroxidise, glutathione, vitamin C and also vitamin E on analysis of liver and kidney samples. The same organs, when analyzed on treatment with the methanolic extract of A. calamus revealed a reversal of these decreased values to normal range. Conclusion; These results thus Indicate the potential use of A. calamus for anticancer studies although the main phytoconstituent in the extract obtained is to be further analyzed.
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    ANTIOXIDANT AND ANTI CLASTOGENIC POTENTIAL OF PIPER LONGUM L.
    (2015) Santhy, K S
    Objective; Presentstudy was aimed to evaluate the antioxidant and anti clastogenic potential of methanol extractor Piper longum L (MEPL). Methods: Chromatographic analysis was carried out using thermo GC-Trace Ultra Ver; 5.0 GC-MS. Antioxidant activities were assessed by DPPH free radical scavenging assay and reducing power assay. Based on the antioxidant activity, micronuclei formation in peripheral blood lymphocytes was analyzed. The protection afforded by Piper longum L against the cytotoxicity of peripheral blood lymphocytes were confirmed by the micronucleus (MNj assay. Results: The GC-MS analysis provides different peaks determining the presence of eighteen phytochemical compounds with different therapeutic activities. The methanol extract at a concentration of 40 pg/ml showed the highest antioxidant activity by DPPH assay (68.42%] comparable to standard, ascorbic acid (73,68%]. The reducing power observed was in the order of 40 pg/ml>20 pg/ml>10 pg/ml. MEPL treatment decreased the frequency of MN in a concentration dependent manner. Conclusion: A substantial amount of bioactive components are present in Piper longum L. A good correlation of the antioxidant capacity of the plant was established by different assay methodologies. MN test confirmed the anti clastogenic potential in peripheral blood lymphocytes.
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    Antioxidant and Antimicrobial Activities of Hygrophila schulli (Buch.-Ham.)
    (2016-04) Thulasi Lakshmi, K; Santhy, K S
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    ANTIOXIDANT AND CYTOTOXIC EFFECTS OF METHANOL EXTRACTS 0¥ AMORPHOPHALLUS PAEONIIFOLIUS NI
    (2015) Santhy, K S
    Objectives; To identify the bioactive compounds present in Amorphophallus paeoniifolius and to evaluate the antioxidant and cytotoxic property of the plant extract. Methods; Preliminary phytochemical analysis was carried out to determine its major chemical constituents such as alkaloid.s, sterols, flavonoids, terpenoids, phenolic compounds, saponins, carbohydrates, glycosides, and tannins. Bioactive compounds were identified by gas chromatography - mass spectrometry (GC/MS) analysis. Antioxidant activity of the methanol extract were determined by two different methods viz., 2,2-diphenyl-2-picryl hydrazyl hydrate radical scavenging assay and reducing power assay. Furthermore, the extracts were tested for their ability to kill the proliferative cells (MCF-7) by (3-[4,5-dimethyl-thiazol-2-yl]-2,5-diphenyltetrazolium bromide) (MTT) assay. Results; Ten pharmacology important compounds were identified by GC/MS analysis. The higher antioxidant potential of the extracts was observed in both assays. The appreciable free radical scavenging activity of the extracts might be attributed to the secondary metabolites such as alkaloids, flavonoids, tannin.s, phenols, and sterols present in the extract. The results of MTT a,ssay indicated a significant growth inhibition at a low concentration of ICju values (51.07 pg/ml). Conclusion; The present study is an evidence to support the usage of the plant which possesses several antioxidant principles for medicinal uses.
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    Antitumor activity of methanolic extract of C yclespeltata
    (2015) Santhy, K S
    Phytocompound based drugs are soon gaining popularity as alternates for treatment of chronic and serious illness. Cydea peltata has largely been classified as medicinal plant and used to treat various illness. Soxhlet based extraction of the plant was carried out using various solvents and subjected to chromatographic analysis to understand the variety of phytoconstituents present in the crude extract of the plant. Invitro and invivo testing for anticancer activity of the methanolic extract of the plant revealed potent significance among the haematological and cytotoxic parameters. The results, thus, indicate efficacy for anticancer activity although the main constituent of the crude extract is yet to be established.
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    Assessment of an I Icancer Activity of Solanvm Torvljm L. Nanoparticles
    (2015) Santhy, K S
    In recent years, metal nanoparticles li.ive received considerable attention III pharmaceutical applications. I’hese n.I noparticles can be synthesized either I hcmically or biologically. Biological synthesis of nanoparticles is less time I onsuming, less Mostly and more eco Inendly. In the present study, the green synlhesis of selenium nano particles from the Solanum torvum fruit extract has been tallied out and characterized by UV-Vis sfx'clra, SEM and FTIR analysis. The anliniicrobial activity of the synthesized ScNI’s against various pathogens and also the cytotoxic activity against MCF-7 breast I .nicer cell line were determined. Solanum u >1 1 um fruits (sundaikkai) were collected in November and December 2013 from the local areas of Coimbatore. The functional groups were observed in the sample tilling FTIR analysis. The SEM image was analyzed to confirm the particle size and .tpjiearance of club formed at 93nm. The itniimicrobial activity was screened against I-coll. Bacillus sp.. Pseudomonas sp. and Kh'hsella sp. The maximum activity occurs at 2mM concentration of selenium naiioparticles. Exposure to increasing Concentration of SeNPs showed dosedependent cytotoxicity on MCF-7 cells.These results throw light on the potent anticancer activity of Solanuin torvum mediated selenium nanoparticles.
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    Challenging Issues and Technological Approaches in Medicine
    (2016) Santhy, K S
    Ethnobotanical alternates for synthetic allopathic drugs are soon gaining importance in the treatment of leases due to their Fewer side eflects. Acorus calamus has been widely used globally due its richness in antioxidant frtoconstituents in the treatments yielding beneficial functions. The methanol extract obtained by soxhlet faction was screened for acute toxicity studies on DAL induced cells. Accordingly 100 and 200 mg/kg body ight were taken as low and high dose of MEAC for the histopathological studies on liver and kidney.In the (sent study the damage induced by DAL cells in the liver sections were partially reversed by the A. calamus fact treatment. The kidney histopathology micrograph of DAL induced mice showed marked tubular and bmerular damage. This damage is regenerated by the treatment with methanol extract of A.calamus. The protective teci of A.calamus against liver and renal cell injury induced by DAL cells might be attributed to its antioxidant pion.
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    CHEMICAL CHARACTERISATION OF ALPI/VIA GALANGA (L.) WILLD BY GC-MS, XRD, FTIR AND UV-VIS SPECTROSCOPIC METHODS
    (2015) Santhy, K S
    Objective: Present study was aimed to produce the chemical profile of Alpiniagalanga (L.) Willd by various analytical methods. Methods: The sample extracted with methanol were screened for their volatile organic constituents using a Shimadzu QP-2010 PLUS gas chromatograph coupled to a mass spectrometer (GC-MS) instrument (Shimadzu Corporation, Japan). Then the sample was analyzed for the identification of unknown crystalline materials and determination of crystal structure using a PANalytIcal X'PERT PRO X-ray diffractometer. Finally the extracts were examined under visible and UV light for the proximate analysis. FTIR method performed on IR AFFINITY-1 Spectrophotometer and UV-1700 Spectrophotometer detects characteristic peaks in the visible range. Results: Forty significant compounds were identified in A. galanga by gas chromatograpby-mass spectrometry method. XRD pattern gave three prominent diffraction peaks at 25 position (15.39 “, 17.42° and 31.32°) results in d-spacing value 5.75, 5.08 and 2.85 A; confirms the presence of Cu, Si and Pb elements. FTIR analysis confirmed the presence of alkanes, amides, carboxylic acids, epoxides, alcohols, aliphatic amines, aromatics and phenol compounds, UV Vis spectrum profile showed the peaks at 358,268 and 224 nm with the absorption of 0.590,1.199 and 2.752. Conclusion: The information regarding the characterization and quantification may be useful in assessing the genotoxicity of the plant material and can be recommended for implementation in tbe official pharmacopeias.
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    Cytotoxic properties of Acorns calamus in MCF -7 breast cancer cells
    (2013) Santhy, K S
    In this study cytotoxic effect of Acorus calamus extract was evaluated on breast carcinoma (MCF-7) cell lines. MCF-7 cells were cultured in DMEM medium and incubated with different concentrations (18.75, 37.5, 75, 150 and 300 |tg/ml) of Acorus calamus methanol extract. Cell viability was assessed by MTT assay. Acorus calamus decreased cell viability in malignant cells in a concentration dependent maimer. The IC50 values in MCF-7 cells were determined as 52.07 pg/ml. It may be concluded that Acorus calamus can cause cell death in MCF-7 cancer cells which can be considered as a promising chemotherapeutic agent in breast cancer treatment.
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    Drug Based Computational Analysis For Initial Stages Breast Caneer with Compounds Obtained from Acorus Calamus
    (2015) Santhy, K S
    The present research on drug development, this study progressed to find the drug target for breast cancer from the plant compound Acorus calamus. Analysis by GCMS of the extract revealed 14 compounds that were consequently taken in for docking studies using highly influential proteins such as BRCAl, BRCA2, PTEN, HER2, CHEK2, ERBbl, ATM in order to check the potential of the binding affinity of the compound. Out of 98 complexes docked with Schrodinger Glide module, four complexes such as ERBbl interacted with [(2R)-2-[(lS)-l-hexadecanoyloxy-2-hydroxyethyl]-4-hydroxy-5- oxo-2H-furan-3-yl] hexadecanoate, PTEN interacted with Tetradecanoic Acid, ATM interacted with Tetradecanoic acid and CHEK2 interacted with 2-hydroxy-6-undecylbenzoic acid showed highest Glide score o f -7.17, -5.92,-4.56, -5.21 correspondingly. Further, Molecular Dynamics Simulation for the protein complex of ERBb2 done using Macromodel module for 1 nanosecond revealed the protein is feasible deviation and fluctuation map from Root mean square calculations. Based on this study, we can conclude that the ErBB 1 protein can be a good target for breast cancer diseased pathway and can be considered that [(2R)-2-[(lS)-l-hexadecanoyloxy-2-hydroxyethyl]-4-hydroxy-5-oxo-2H-furan-3-yl] as a ligand can act as a good inhibitor for breast cancer.