Browsing by Author "Annapurani, S"
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Item Antioxidant Activity o f Phenolic Fractions o f 7ermina7ia Catappa in Ela Propagated Swiss Albino Mice(2011) Annapurani, SThis investigation aims to evaluate the antioxidant potential of Phenolic fractions of Terminalia catappa in ELA propagated Swiss albino mice. The levels of enzymic antioxidants such as catalase, superoxide dismutase and glutathione peroxidase and non enzymic antioxidants such as vitamin A, vitamin E and reduced glutathione were increased on administration with phenolic fractions of Terminalia catappa in ELA induced mice. This result suggests that phenolic fractions of Terminalia catappa possess antioxidant activity.Item Antioxidant Status in the Mice Treated With the Protein Fraction of the Grass Cynodondactylon(2010) Annapurani, SCells are equipped with enzymatic and non enzymatic antioxidative mechanism that play an important role in elimination o f free radicals SOD, Catalase, Glutathione peroxides and reductase involved in the clearance o f superoxide and HjOj.Hence the present study was carried out to evaluate the antioxidant levels and observed catalase (60.21U/mg protein), superoxide dismutase (4.49U/mg protein), glutathione peroxidase (3.3U/mg protein), glutathione reductase (10.8U/mg protein)and glutathione -s- transferase (2083.32U/mg protein) in the liver ofthe experimental mice treated with protein fraction o f Cynodon dactylon. The results were found to be statistically significant when compared to their controls.Item Antioxidative Activity of Methanolic Extract of Gloriosa Superba Seed, Tuber and Leaves(2013) Saradha Devi, K M; Annapurani, SMedicinal plants play a key role in the human health care. About eighty percent of the world populations rely on the use of traditional medicine, which is predominantly based on plant materials. The present study was carried out to evaluate the antioxidative activity of methanolic extract of Gloriosa superba seed, tuber and leaves (MEGSSTL). The percentage radical scavenging of MEGSSTL on DPPH and the IC50 (50% inhibitory concentration) value was found to be 38, 52 and 74 pg., hydrogen peroxide scavenging activity of MEGSSTL and the IC50 value was found to be 50, 63 and 68 pg., nitric oxide scavenging activity of MEGSSTL and the ICso value was found to be the 61,71 and 84 pg., hydroxyl radical scavenging ability of MEGSSTL to 50 percent was found to be 70,76 and 85 pg (ICso) ond superoxide radical production in vitro in a dose dependent manner with an IC50 of 58,70 and 75 pg. respectively. The dose dependent percentage scavenging activity of DPPH, hydrogen peroxide, nitric oxide, hydroxyl and superoxide radical by methanolic extracts indicated its antioxidative efficacy.Item Antitumor Activity of Methanolic Extract of Terminalia Catappa Leaves Against Ehrlich Ascites Induced Carcinoma In Mice(2011) Annapurani, STerminalia catappa belongs to the family combretaceae and is popularly known as badam.lt is a well known herb in Ayurvedic system of medicine The aim of the present study is to evaluate the effect of methanolic extract of leaves of Terminalia catappa against Ehrlich ascetic Lymphoma (ELA) in Swiss albino mice.The tumor was induced in mice by intraperitoneal injection of ELA cells (lX106cells/mice). Methanolic extract of Terminalia catappa was administered to the experimental animals at a dose of 75pg/kg/day after 24 h of tumor inoculation. The antitumor effect of extract was evaluated by assessing in vitro cytotoxicity, increase in lifespan, hematological parameters and liver enzymes. The methanolic extract brought back the altered levels of the hematological parameters and liver enzymes. Thus the present study revealed that methanolic extract of Terminalia catappa possessed significant antitumor activity.Item Biochemical Profile of Calotropis procera(2010) Saradha Devi, K M; Annapurani, SItem Exploitation of Cryptococcus Neoformans Isolated From Cow Dung In Bioremediation of Medical Waste(2019-04) Akshaya Ravindran; Annapurani, SItem Hepatic antioxidative potential of ethyl acetate fraction of Cynodon dactylon in Balb/c mice(2011) Annapurani, SA study was undertaken to evaluate the antioxidative potential of ethyl acetate fraction of Cynodon dactylon in Balb/c mice. In this present study mice were divided into the two groups and each group containing 6 mice. Group I mice were orally administered with 70 pi of DMSO and are the vehicle control for ethyl acetate fraction. Group II mice were injected with ED50 70 pi of ethyl acetate fraction of C. dactylon. The activity of enzymic antioxidants (U/ mg of protein) such as catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GPX) were found to be significantly high in ethyl acetate fraction treated mice when compared to the control mice. The levels (g liver tissue) of nonenzymic antioxidants such as vitamin A, vitamin C, vitamin E and reduced glutathione in the ethyl acetate fraction treated mice (Vitamin A 158.224 pg, Vitamin C 7.442 pg, Vitamin E 5.383 pg and reduced GSH 489.283 nmoles) was found to be significantly higher than that found in control mice. These results suggest that ethyl acetate fraction of C. dactylon has very good antioxidant and hepatic protective effect of normal oxidative stress in Balb/c mice. Currently, the importance of oxidative stress in the pathophysiology of many human disorders has been highlighted, thus use of this plant as an herbal medicine is highly recommended. Hereafter, trials to establish efficacy and optimum dosage of the present herbal product for treating human chronic diseases with pathophysiology of oxidative stress are essential.Item Icewound Healing Activity of Flavonoid Fraction of Cynodondactylon in Swiss Albino Mice(2012) Annapurani, SThe aim of the present study is to evaluate the wound healing activity of flavonoid fraction of Cynodon dactylon in Swiss albino mice. The wound healing property of the fraction was studied in excision wound which was inflicted by cutting away 0.5cm standard full thickness of wound was created on the predetermined dorsal area. The flavonoid fraction of Cynodon dactylon were applied externally daily on the excised wound area for 8 days. This fraction facilitates the healing process as evidenced by increase in collagen and protein and decrease in lipid peroxide in granulation tissue. Thus, the enhanced wound healing may be due to free radical scavenging activity of the flavonoid fraction.Item In Silico and Pharmokinetic (Adme) Analysis for Selective Inhibitors of Tubulin as Potential Cure for Cancer(2010) Annapurani, SMicrotubules are extremely important in the process of mitosis, during which the duplicated chromosomes of a cell are separated into two identical sets before cleavage of the cell into two daughter cells. Their importance in mitosis and cell division makes microtubules an important target tor anticanccr drugs. y\ new class of simple synthetic antimitotic compounds based on 2-aroyliridolcs was drawn using MAESTRO and CHEMSKF/rCl!. flic crystal structure of TUBULIN was used as a target structure. It was obtained from RCSB Protein Data Bank with the PDB ID; IZ2B. High Throughput Virtual Screening, and Induced Fit Docking studies were carried out using GLIDE. Docking stuH>es was also carried out using GOLD. Interactions were noted for both ligand and synthetic compounds with the target protein. Energy values, GLIDE and GOLD scores were^PNIE studies were carried out '■ "To’rTnose compounds and its activity was predicted using PASS prediction. Based on this, 3-Benzyloxypheiiyl- (5-metlioxy-lpiiciiylsulfonyl- 1 U-2-indolyl)-inetliaiioi was found to be potent inhibitor for tubulin. We conclude, that 2-aroylindoies constitute an interesting new class of antitubulin agents with the potential to be clinically developed for cancer treatment.Item Influence of Iron and Copper on invitro culture of Withania somnifera(2018-04) Kousalya, R; Annapurani, SItem Molecular docking studies of 4 - Piperidinopyridine and 4 - Piperidinopyrimidine derivatives as 2,3 - Oxidosqualene Cyclase inhibitors(2011) Annapurani, SLanosterol synthase belongs to the large family of oxidosqualene cyclases (OSCs), eukaryotic enzymes that catalyse the cyclization of 2,3-oxidosqualene(OS) into different cyclic compounds. A novel series of 4-piperidinopyridines and 4- piperidinopyrimidines showed potent and selective inhibition of 2,3-oxidosqualenc cyclase-lanosterol synthase (OSC). The piperidinopyrimidine OSC inhibitors have a significantly lower pl<.(a) than the corresponding pyridine .This indicates that other novel OSC inhibitors may be found in analogues of this series across a broader pl<.(a) range (6.0-9.0). These series may yield novel hypccholesterolemic agents for the treatment of cardiovascular disease. Docking analysis of GOLD and GLIDE were performed to predict the binding properties of disease related targef protein OSC with 4-Piperidinopyridine and 4- Piperidinopyrimidine derivatives as inhibitors. Virtual Screening has been done for all derivatives (inhibitors) and the ligands were chosen for induced fit docking based on their binding affinity, glide energy and glide score. The results revealed that the compound 25 (I-(4-Pyrimiclinyl)-4-(I-(4-brninopl]unyl -siilfonyl)piperazin-4ylcarbonyl)piperidine) with more interaction and scores may afford novel hypocholcstorolemic agents for the treatment of cardiovascular disease.Item Non Enzymic Anti Oxidants ( Vitamin A, E «& C ) in the Liver of the Mice Treated With Protein Fraction of Cynodon Dactylon(2010) Annapurani, SProtein fraction of Cynodon dactylon, a plant with various medicinal properties, has been investigated for its non enzymic anti oxidants-vitamin A, vitamin E and vitamin C in the Swiss Albino mice. Results depicted that thete is a statistically significant increased levels. The non enzymic antioxidants like vitamin A, E and C content were found to be 19.75 pg) g tissue, 24.25 m g/g tissue and 3.08 mg /g tissue respectively in the liver of the experimental mice when compared to their corresponding vehicle controls. These observations clearly indicate that these non enzymic anti oxidants may be one of the factors responsible for the anti carcinogenic effects.Item Research Journal of Pharmaceutical, Biological and Chemical(2012) Annapurani, SPhytochemical constituents of methanolic extract of Gloriosa superba seed, tuber and leaves (MEGSSTL) were assessed by preliminary phytochemical screening analysis. Phytochemical analysis of the Gloriosa superba seed showed the presence of Carbohydrates, Alkaloids, Glycosides, Flavanoids, Steroids, Terpenoids and Phenolics; Gloriosa superba tuber showed the presence of Carbohydrates, Alkaloids and Flavanoids; Gloriosa superba leaves showed the presence of Carbohydrates, Alkaloids, Flavanoids, Steroids and Terpenoids.Item Studies on Immunomodulatory Activity of Flavonoid Fractions of Terminalia Catappa in Swiss Albino Mice(2012) Annapurani, STo investigate the immunomodulatory activity of flavonoid fractions of Terminalia catappa in Swiss albino mice. The flavonoid fraction of T.catappa was administrated intraperitonealy at a dose of ED50 to healthy albino mice. Assessment of immunomodulatory activity was carried out by assessment of neutrophil adhesion and phagocytic index. Intraperitonealy administered Tcff showed a significant increase in neutrophil adhesion and phagocytic index.This study demonstrates that the Tcff shows immunomodulatory effect.