Hepatic antioxidative potential of ethyl acetate fraction of Cynodon dactylon in Balb/c mice
No Thumbnail Available
Date
2011
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
A study was undertaken to evaluate the antioxidative potential of ethyl acetate fraction of Cynodon
dactylon in Balb/c mice. In this present study mice were divided into the two groups and each group
containing 6 mice. Group I mice were orally administered with 70 pi of DMSO and are the vehicle control
for ethyl acetate fraction. Group II mice were injected with ED50 70 pi of ethyl acetate fraction of C.
dactylon. The activity of enzymic antioxidants (U/ mg of protein) such as catalase (CAT), superoxide
dismutase (SOD) and glutathione peroxidase (GPX) were found to be significantly high in ethyl acetate
fraction treated mice when compared to the control mice. The levels (g liver tissue) of nonenzymic
antioxidants such as vitamin A, vitamin C, vitamin E and reduced glutathione in the ethyl acetate
fraction treated mice (Vitamin A 158.224 pg, Vitamin C 7.442 pg, Vitamin E 5.383 pg and reduced GSH
489.283 nmoles) was found to be significantly higher than that found in control mice. These results
suggest that ethyl acetate fraction of C. dactylon has very good antioxidant and hepatic protective
effect of normal oxidative stress in Balb/c mice. Currently, the importance of oxidative stress in the
pathophysiology of many human disorders has been highlighted, thus use of this plant as an herbal
medicine is highly recommended. Hereafter, trials to establish efficacy and optimum dosage of the
present herbal product for treating human chronic diseases with pathophysiology of oxidative stress
are essential.