Comparative Study on Precipitation Techniques for Protease Isolation and Purification from Labeo Rohita Viscera

dc.category	Journal Article
dc.contributor.author	Anitha Subash
dc.date.accessioned	2017-03-08T23:03:21Z
dc.date.available	2017-03-08T23:03:21Z
dc.date.issued	2012
dc.department	Biochemistry	en_US
dc.description.abstract	Precipitation techniques play a vital role in the industrial extraction of enzymes. The present study aimed to extract the proteases from the viscera of Labeo rohita (commonly called Rohu) and to compare the precipitation techniques for the isolation and purification of the enzyme. The enzyme is usually discarded as tons of waste during processing. Hence, a trial has been carried out to isolate the protease enzymes from viscera of the fresh water fish Labeo rohita. The proteases were precipitated with ammonium sulfate, ethanol, and acetone. Acetone precipitation was found to be the best option for the recoveiy of enzymes (54%) from the viscera o f Rohu, and two caseinolytic protease bands were shown in the Zymogram. The precipitates with highest proteolytic activity were further subjected to dialysis, and their molecular weight was determined by sodium dodecyl sulfate polyaciylamide gel electrophoresis (SDS-PAGE).	en_US
dc.identifier.uri	https://ir.avinuty.ac.in/handle/avu/1999
dc.lang	English	en_US
dc.publisher.name	Journal of Aquatic Food Product Technology	en_US
dc.publisher.type	International	en_US
dc.title	Comparative Study on Precipitation Techniques for Protease Isolation and Purification from Labeo Rohita Viscera	en_US

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