Comparative Study on Precipitation Techniques for Protease Isolation and Purification from Labeo Rohita Viscera
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Date
2012
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Abstract
Precipitation techniques play a vital role in the industrial extraction of enzymes. The present study aimed to extract the proteases from the viscera of
Labeo rohita (commonly called Rohu) and to compare the precipitation techniques for the isolation and purification of the enzyme. The enzyme is
usually discarded as tons of waste during processing. Hence, a trial has been carried out to isolate the protease enzymes from viscera of the fresh water
fish Labeo rohita. The proteases were precipitated with ammonium sulfate, ethanol, and acetone. Acetone precipitation was found to be the best option
for the recoveiy of enzymes (54%) from the viscera o f Rohu, and two caseinolytic protease bands were shown in the Zymogram. The precipitates with
highest proteolytic activity were further subjected to dialysis, and their molecular weight was determined by sodium dodecyl sulfate polyaciylamide gel
electrophoresis (SDS-PAGE).