Inverse Electron Demand Diels Alder Reaction of Pyrido[1,2-a]pyrimidine 2-ones - Experimental and Theoretical Investigation and Antibacterial  Activity of Synthesized Adducts

Abinaya A; Dr. V.Sharulatha

Inverse Electron Demand Diels Alder Reaction of Pyrido[1,2-a]pyrimidine 2-ones - Experimental and Theoretical Investigation and Antibacterial Activity of Synthesized Adducts

dc.contributor.author	Abinaya A
dc.contributor.author	Dr. V.Sharulatha
dc.date.accessioned	2025-06-20T05:03:19Z
dc.date.available	2025-06-20T05:03:19Z
dc.date.issued	2023-03
dc.description.abstract	The IEDDA reaction of diene 2-oxo-2H-pyrido[1,2-a] pyrimidin-3(4H)-ylidiene acetic acid with various electron rich dienophiles in DMF medium yielded moderate to good yields in the presence of Lewis acid catalyst indium (III) chloride. FT-IR, 1H NMR, 13C NMR, and Mass Spectroscopy investigations were used to characterize and confirm the structures of all the synthesized adducts. Except for 3-butoxy-9-methyl-2,3,4,5 tetrahydropyrano[2,3-d]pyrido[1,2-a]pyrimidine-4-carboxylic acid (5) and Methyl-2-Oxo-1 Phenyl-1,2,3,3a,4,5-hexa hydropyrazolo[1,5-b]Pyrido[1',2':1,2]Pyrimido[5,4 e][1,2]Oxazine-4-Carboxylic Acid (7) which showed 1:1 and 6:4 ratio and for other adducts the ratio was found to be 6:1, supporting the endo rule. The analysis of the LUMO and HOMO energies of diene and dienophiles helped to prove the IEDDA reaction pathway. NBO charges computed using DFT techniques gave justification for the regioselectivity provided by the reaction. The synchronous nature of the transition states and intrinsic reaction coordinates of the reaction of diene 2-oxo-2H-pyrido[1,2-a] pyrimidin-3(4H) ylidiene acetic acid with dienophiles butyl vinyl ether and 1-methyl-1-cyclohexene by DFT method explained the observed diastereoisomeric ratio of 1:1 and 6:1 endo and exo ratio respectively. All synthesized compounds were tested for antibacterial activity, which showed moderate activity, however the compound 3a-methyl 2-oxo-1-phenyl-1,2,3,3a,4,5-hexahydropyrazolo[1,5-b]pyrido[1',2':1,2]pyrimido[5,4 e][1,2]oxazine-4-carboxylic acid was shown to be more powerful in both in-silico and in vitro investigations.
dc.identifier.uri	https://ir.avinuty.ac.in/handle/123456789/17526
dc.language.iso	en
dc.subject	Chemistry	en
dc.title	Inverse Electron Demand Diels Alder Reaction of Pyrido[1,2-a]pyrimidine 2-ones - Experimental and Theoretical Investigation and Antibacterial Activity of Synthesized Adducts
dc.type	Learning Object