Utilization of Visceral Waste of Ribbon Fish {Trachjpterus Tracijypterus) for the Extraction, Purification and Characterization of Protease

Simple item page
dc.categoryJournal Article
dc.contributor.authorAnitha Subash
dc.date.accessioned2017-03-07T22:28:21Z
dc.date.available2017-03-07T22:28:21Z
dc.date.issued2011
dc.departmentBiochemistryen_US
dc.description.abstractAbstract: Rihhon ftsli (I'radiiptcrus trachyptcrus) i\ one of the mast commonly constimctlfisli in Coimbatore Ribbon fish possesses all the characteristics o f fish iivinp in very preat depth. Their fins especialiy and the membrane connectinp them are o f a very delicate and brittle structure. There is some potential fo r gaininp more value from fish waste. It is rich in valuable minerals, enzymes, pipments and favours that are required by many industries includinp food, apriculture, aquaculture and pharmaceuticals industries. Fish waste can also he utilised in the production o f orpanic fertilisers and composts, which have sipnificant benefits over chemical-based products. The aim of the present study was to utilize the visceral waste of ribbon fish (Trachipterus trachyptenis) for the extraction, purification and characterization o f protease. The wastes o f the fish Trachipterus trachyptenis were-coUected, cleaned, Itomopenizc’d, precipitated with ,4111111011111111 sulphate o f varyinp concentrations (0- S0%), purified by dialysis, Sephadex-G100 chromatapraphy, molecular weiphi determined by SD.S - PAGE, the presence o f protease confirmed by /ymopraphy and its activity assayed and atialyzed. The activity was found to be hiphest at the alkaline pH 7.H and maximuin protease activity remained at 50"C.en_US
dc.identifier.urihttps://ir.avinuty.ac.in/handle/avu/1942
dc.langEnglishen_US
dc.publisher.nameJournal of Cell and Tissue Researchen_US
dc.publisher.typeInternationalen_US
dc.titleUtilization of Visceral Waste of Ribbon Fish {Trachjpterus Tracijypterus) for the Extraction, Purification and Characterization of Proteaseen_US
Files
Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
GENERATION AND ANALYSIS_050.pdf
Size:
3.22 MB
Format:
Adobe Portable Document Format
Download
Collections
Journal Article