Utilization of Visceral Waste of Ribbon Fish {Trachjpterus Tracijypterus) for the Extraction, Purification and Characterization of Protease
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Date
2011
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Abstract
Abstract: Rihhon ftsli (I'radiiptcrus trachyptcrus) i\ one of the mast commonly constimctlfisli
in Coimbatore Ribbon fish possesses all the characteristics o f fish iivinp in very preat
depth. Their fins especialiy and the membrane connectinp them are o f a very delicate and
brittle structure. There is some potential fo r gaininp more value from fish waste. It is rich
in valuable minerals, enzymes, pipments and favours that are required by many industries
includinp food, apriculture, aquaculture and pharmaceuticals industries. Fish waste can
also he utilised in the production o f orpanic fertilisers and composts, which have sipnificant
benefits over chemical-based products. The aim of the present study was to utilize the
visceral waste of ribbon fish (Trachipterus trachyptenis) for the extraction, purification and
characterization o f protease. The wastes o f the fish Trachipterus trachyptenis were-coUected,
cleaned, Itomopenizc’d, precipitated with ,4111111011111111 sulphate o f varyinp concentrations (0-
S0%), purified by dialysis, Sephadex-G100 chromatapraphy, molecular weiphi determined by
SD.S - PAGE, the presence o f protease confirmed by /ymopraphy and its activity assayed
and atialyzed. The activity was found to be hiphest at the alkaline pH 7.H and maximuin
protease activity remained at 50"C.