Glycyrrhiza glabra - a Natural Panacea against Diarrhoea and Cancer
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Date
2016
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Abstract
The major food borne outbreaks of diarrhoea! diseases are caused by microbes such as Escherichia coli.
Salmonella enterica and Shigella dysenteriae. The present study was conducted to develop nanoparticle
coatings from the medicinal plant, against diarrhea and carcinoma. The GC/MS of nanoparticles
reveals the following active phyto constituents namely 9-octadecenal, tetradecanoic acid, oleic Acid, z, z 2, 5-
Pentadecadien-1 -ol, cis-9,10-epoxyoctadecan-1 -ol and n-hexadecanoic acid. The antioxidant and thin layer chromatography
of Glycyrrhiza glabra nanoparticles showed a maximum of 520 pg and phenolic compounds. The
antimicrobial activity were tested by disc diffusion showed microbial inhibition of 11,15 and 14 mm against E.coli
(MTCC 40), S.entericaifATCC 3219) and S.dysenteriae (PSGIMS&R) respectively. Therefore, commercially
available food packages such as PET bottles, zip lock covers and infant feeding bottles were coated with nanoparticles
and tested for shelf-life for ten days. In nanoeoated PET bottles and ziplock covers stored with tomato puree
showed that till b* day, there was no microbial growth. On the 10'*' day, 57,68 and 88 percentage of microbial
inhibition was observed in PET bottles against E.coli (MTCC 40), S.enterica (MTCC 3219) and S.dysenteriae
(PSGIMS&R) respectively. In nanoeoated zip lock covers showed 38,63 and 83 percentage of microbial inhibition
was observed against diarrhoeal pathogens. Nanoeoated feeding bottles, showed a maximum microbial inhibition
(84percentage) against diarrhoeal pathogens. Gel electrophoresis showed DNA damage to the Hela and Liver
carcinoma cell lines and 50 and 48 per cent apoptosis in the two cell lines respectively. Therefore, Glycyrrhiza
glabra nanoparticles are effective in diarrrhoea prevention and cancer treatment.