ANTIMUTAGENIC EFFECT OF GREEN TEA EXTRACTS IN REVERSE MUTATION ASSAY
No Thumbnail Available
Date
2013
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
In the present investigation, antimutagenic effect of petroleum ether, chloroform, methanol and
water extracts of green tea was evaluated in Salmonella typhimurium TA-98 and TA-100 strains.
Well known mutagens like sodium azide and daunomycin were added at a concentration of lOpI
and 6pi per plate respectively resulted in the induction of histidine revertant colonies. However
addition of 10 pi of petroleum ether, chloroform, ethanol and water extracts of green tea to 10 pi
of sodium azide and 6 pi of daunomycin treated plates resulted in the inhibition in the number of
histidine revertant colonies. Furthermore, supplementation with all the four extracts of green
tea at a concentration of 10 pi per plate respectively in the presence of S9 fraction also led to
significant inhibition in sodium azide and daunomycin induced colony formation. The
antimutagenic activity of ethanolic extract of green tea was found to be higher than that of the
other extracts. Hence the study revealed that green tea has protective efficacy in sodium azide
and daunomycin induced mutagenicity in the test microbial system.