Assessment of Free Radical Scavenging Activity and Antioxidant Potential of the TLC separated Fractions of Rhinacanthus nasutus Root Extracts
No Thumbnail Available
Date
2012
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
The root powder was shade dried and
powdered and subjected first to extraction by series of
solvents on increasing polarity (Petroleum ether,
Benzene, Chloroform, Ethyl acetate, Methanol and
Water). Then the extracts were subjected to TLC and
the spots obtained were eluted and used for the
further antioxidant study. DPPH, ABTS, Hydroxyl
radical, Hydrogen peroxide radical scavenging assays
and determination of inhibition of in vitro nitric oxide
and superoxide generation were determined to
evaluate the free radical scavenging activity of the
extract. The total antioxidant potential of the extract
was studied by phosphomolybdenum assay and
•
reduction potential assay. The results of our experiment
showed that plant extract has highest radical
scavenging activity.