Browsing by Author "Anitha Subash"
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- ItemAnalysis of Antinutritional Substances and Improvement in the Protein Digestibility of Sword Bean {Canavalia gladiata Jacq.) Seeds during Processing(2011) Anitha SubashCost-effective processing methods were studied to remove the antinutritional compounds in red and white sword bean (SB) [Canavalia gladiata Jacq.) seeds. Soaked and soaked and autoclaving effectively reduced the total free phenolics (69-76%), tannins (53-71%), L-Dopa (73-78%), Phytic acid (55-80%), oligosaccharides such as raffinose (74-80%), Stachyose (73-84%) and verbascose (79-88%), trypsin inhibitor activity (57-77%) and also improvement in the in vitro protein digestibility (90-98%) in white and red SB seeds. Adoption of this processing method will enhance the utilization of SB and provide alternative/additional protein source for both human beings and animals.
- ItemAntimicrobial Compounds Production by i®i'eM(io/7zo/za5' fluorescens and Bacillus subtilis(2012) Anitha SubashIn the present study, ten strains of Pseudomonas fluorescens and ten strains of Bacillus subtilis were isolated from the soil sample. The production of antimicrobial ,cornpounds such as HCN,;.sfd,er'ophQres, salicylic .acid and indole acetic acid by the isolates were estimated. Among thelsplates A.L)PF3,. AUES.2 'and AUBS.8, showed maximum production compared to the other isolates. Thus from the-above study, it can be cdncluded that effective isolates can be.used, as:, biocontrol agents;
- ItemBiochemical Studies in Vermicomposts from Cow Dung(2010-05) Sindhiya , D; Anitha Subash
- ItemBiocomposting of Tapioca Solid Waste with Selected Earthworms and Microbes(2011-05-05) Malathi, R; Anitha Subash
- ItemBIOLOGICAL MATURITY AND CHEMICAL STABILITY OF MICROBIAL INFLUENCED TAPIOCA SOLID WASTE VERMICOMPOST(2010) Anitha SubashTapioca solid waste (TS\V), a major biodegradable solid waste having high amounts of organic matter, act as a better source and cow dung (CD) used as bulking material in the proportion of 3:1 for polyculture earthworm composting. Ain; of the present study was to evaluate the biological maturity and chemical stability (vermicompost aging) by analyzing the humic acict'level, microbial biomass, earthworm biomass, cocoon formation and worm mortality rate in microbial (Trichoderma viridae and Bacillus polyntyxa) aided TSW vermicomposting, because these parameters can control the quality of the resulting vermicompost. Analysis of the composts in control, microbes, earthworms and earthworms with microbes treated media were done on the 2S''‘, SO"' and 75"' day.s. Significant changes were observed in all media except control. Significant ' changes, such as: 1. increased'iinmic acid content, 2. increased microbial biomass (COj up to SO''' day then decline on the 75''' day, 3. decreased earthworm biomass, 4, increased earthworm biomass and 5. cocoon formation, were recorded. The yield of the final compost was high in earthworms with microbes treated medium. Thus, our results reveal that the use of selected microorganisms with polyculture earthworms enhances the composting process and chemical stability of Tapioca solid waste vermicomposting.
- ItemCHARACTERISATION OF TANNERY SOLID WASTES AT DIFFERENT STAGES OF LEATHER PROCESSING AND VERMICOMPOSTING AS A METHOD OF DEPOLLUTING THE WASTES(2010) Anitha SubashFour types of wastes were collected from different stages of leather processing namely, LDB-BT, LDB-AT, PW-BT and CTW-ACR and mixed with equal amounts of vegetable wastes and vermicomposted using local species of earthworms and then analyzed. The results show an increase in pH, EC and decrease in COD, TDS, phosphorus, potassium, sodium, calcium, and magnesium in the vermicompost.
- ItemCharacterization, Immobilization and Applications of Extracellular Protease from Bacillus sp. ASASBT isolated from Termite Soil(2019-09) Sujatha, A; Anitha Subash
- ItemCoagulation Proteins in Rats During Hypoxia Reversal by Oxygen Nanobubbles(2023-05) Akalya Bai, S K; Anitha Subash
- ItemComparative Study on Precipitation Techniques for Protease Isolation and Purification from Labeo Rohita Viscera(2012) Anitha SubashPrecipitation techniques play a vital role in the industrial extraction of enzymes. The present study aimed to extract the proteases from the viscera of Labeo rohita (commonly called Rohu) and to compare the precipitation techniques for the isolation and purification of the enzyme. The enzyme is usually discarded as tons of waste during processing. Hence, a trial has been carried out to isolate the protease enzymes from viscera of the fresh water fish Labeo rohita. The proteases were precipitated with ammonium sulfate, ethanol, and acetone. Acetone precipitation was found to be the best option for the recoveiy of enzymes (54%) from the viscera o f Rohu, and two caseinolytic protease bands were shown in the Zymogram. The precipitates with highest proteolytic activity were further subjected to dialysis, and their molecular weight was determined by sodium dodecyl sulfate polyaciylamide gel electrophoresis (SDS-PAGE).
- ItemA comparative Study on the Antioxidant Properties of Unripe and Ripe Fruits of Citrullus colocynthis (Linn.)(2010-10) T Santhiya; Anitha Subash
- ItemEffect of bacterial antagonists against Sclerotium rolfsii, a root rot pathogen of Stevia (Stevia rebaudiana)(2010-10) Prema, S; Anitha Subash
- ItemEffects of processing technique on the nutritional composition and aiitinutrients content of under -utilized food legume Ccinavalia ensiformis L.DC.(2011) Anitha SubashJack Bean (JB) (Canavalia ensiformis) is an indigenous legume promoted in Tamilnadu as a green manure cover crop. It contains high protein but it is under - utilized due to the presence of 3, 4-dihydroxy- Lphenylalanine (L-D6pa) and other anti nutritional compounds. To improve its nutritional potential as a protein source, Jack bean was processed and evaluated for nutritional composition. Effects of processing at different methods like soaking, cooking and autoclaving on the contents of anti-nutritional compounds and crude protein were investigated. Raw beans contained high crude protein (29.8 g/100'). Contents of ether extract; crude fibre and ash were 4.2, 7.37 and 4.48 g/100 ', respectively. Raw whole Jack bean contained L-Dopa (1.7 g/100 ‘) content. Other anti-nutritional compounds included total phenols 3.83 g/100-1, trypsin inhibitor activity (TIA) 378.3 TIU and tannins 82.5 mg/100 '. The present study also evaluated the changes of anti-nutritional factors of Jack bean by subjecting to soaking, autoclaving and cooking. Maximum reduction was observed in TIA, L-Dopa and Total free phenols with autoclaving, while the soaking and cooking decreases the levels of tannins. Autoclaving was more effective method in reducing trypsin inhibitor activity, polyphenols and L-Dopa than the various cooking treatments.
- ItemEfficacy of talc-based fornuilation of Pseudomonas fluorescens on the management of leaf spot disease of Stevia rebaudiana Bertoni(2014) Anitha SubashStevia rebaudiana Bertoni, *3 natural sweetener contains two main sweetest compounds, stcyioside (ST) and rebaudioside A (R-A), tasting about 300 and 450 times sweeter than sucrose, respectively. I ’his commercially important plant also suffers a leaf spot disease caused by thesfungus Alteniaria alternata in various districts of Tamil Nadu, India. In the present study, ten isolates o f fluorescent pseudomonads were evaluated for their ability to control leaf spot in stevia.^(Steviasrebaudiana Bertoni). These isolates were characterized as Pseudomonas fluorescens on,biochemical tests. Among these isolates, P. fluorescens \so\a\e AIJPF'6 .', and AUPF5 showed ’ the maximum inhibition of mycelial gtovA)): o i Alternaria alternata. They also increased plant growth in stevia apart from reducing thedeaf spot incidence under greenhouse condition. The isolates AUPF6 and AUPF5 were further tested for their ability to iinduce production of defense’*related enzymes and, ■chemicals in" plants,'‘ Earlier ■■ and -increased^ activities of phenylalanine ammonia lyase (PAL),' iperoxidase (PO) and polyphenol oxidase (PPO) were observed in ■ P.. fluorescens AUPF6 and AUPF5 pretreated stevia plants challenged with Alternaria i alternata. Moreover, • higher accumulation of phenolics was noticed in plants ipretreated with P. fluorescens isolates AUPF6 and AUPF5 challenged with Alternaria alternata . Thus, the present study shows that in additionito direct antagonism and plantsgrowth-prpinotion, 'induction of defense-related enzymes involved in the phenyl •'‘propanoid pathway collectively contributed to enhance resistance against^ithe \maaion o f Alternaria alternata in sXcvia.
- ItemExtraction, Purification and Characterization of Protease from the Viscera of White Pomfret (Pampus ARGENTEUS)(2008-05) Nirmala Devi , P G; Anitha Subash
- ItemExtraction, Purification and Characterization of Protease from the visceral organ waste of Milk Shark (Rhizoprionodon acutus)(2008-05) Revathi , P; Anitha Subash
- ItemExtraction, Purification and Characterization of Protease from Visceral organ waste of Carangoides malabaricus (Malabar trevally)(2009-04) Jeyashri , G; Anitha Subash
- ItemIdentification Of Molecular Markers For The Diagnosis Of Leukemia(2022-05) Kalaimathi, M; Anitha Subash
- ItemInduction of Defense Related Enzymes and Growth Promoting Traits in Tomato (Lycopersicon Esculentum) Plant(2012-12) S Shobana; Anitha Subash