ANTICANCER ACTIVITY OF Artemisia vulgaris ON HEPATOCELLULAR CARCINOMA (HEPG2)CELLS
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Date
2012
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Abstract
Hepatocellular carcinoma is the ifth leading cancer causing death worldwide. Currently research focuses on the use of medicinal plants for the
treatment of cancer, due to its resistance to chemotherapeutic agents.
Objective: The present study pertains to the use of Artemisia vulgaris as a source of anticancer agent The methanolic extract of the leaves were
tested in vitro against HepG2 [hepatocellular carcinoma) cells for their apoptotic properties in synergism with a standard chemotherapeutic drug,
etoposide.
Methods: Viability of the cells was assessed by various concentrations of tfie extract using MTT. Cytotoxicity was assessed by the LDH assay and
mechanism of cell death was studied by morphological [Giemsa) and various nuclear staining methods (EtBr, PI and DAPI).
Results: The 1C50 value was found to be around O.lmg as determined by MTT assay. LDH cytotoxicity assay showed A. vulgaris leaf extract to be
toxic against the cancer cells, which was further proved by the staining methods.
Conclusion: The results indicated the potential use of Artemisia vulgaris leaf extract as a source of chemotherapeutic substances.