Biochemical Characterization oe Amylase From Aspergillus Niger and its Application in Desizing
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Date
2015
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Abstract
Reduction in pollution and
improvement in the fabric quality can be
achieved using enzymatic treatment.
Aspergillus niger isolated from distillery
effluent spilled soil was selected for the
study due to its maximum amylase activity.
The optimum amylase activity was
observed at the pH nine in glycine sodium
hydroxide buffer at 60"C for 60 minutes and
the enzyme was stable for the first two
hours of incubation. A.niger utilized one per
cent of starch for maximum amylase activity
in medium supplemented with 5g NaCl.
Calcium ions were found to activate the
enzyme activity whereas mercury ions
inhibited the activity to higher extent when
compared to other ions. The enzyme was
found to desize the fabric effectively. The
visual inspection proved all sample to have
good general appearance and better texture
after enzyme treatment. Luster and
whiteness did not show significant change
after treatment. All the samples showed
reduction in stiffness after desizing with
enzymes. Hence, amylases can be explored
in textile sector that requires a wide range
of optimal conditions.