Partial Characterization of Protease from the Visceral Organ Waste of Cobia {Rachycentroii canadum)
No Thumbnail Available
Date
2013
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
Protea.ses are the most important enzymes accounting for approximately 60% of the total industrial enzyme
market. Its isolation from the visceral organ wa.ste of Cobia showed the highest specific activity in the 4 0 - 50 %
ammonium sulphate precipitated sample. Hence, that fraction was dialyzed and was found to have an increase in
the specific activity from 2.0 - 3.2 U/mg. Purification of the dialyzed fractions on Sephadex G-lOO column also
revealed an increase in the specific activity from 3.2 - 3.5 U/mg. The molecular weight of the enzyme was found
to be 32.0 KDa. Zymogram was done to confirm the presence of protease. The optimum pH and temperature of
the isolated enzyme was shown to be 8 and 60"C where the protease activity was found to be the highest.
Keywords: Cobia, alkaline protease, fish waste, enzyme