Optimization of Protease Production by Bacillus subtilis Isolated from Mid Gut of Fresh Water Fish Labeo rohita
| dc.category | Journal Article | |
| dc.contributor.author | Anitha Subash | |
| dc.date.accessioned | 2017-03-07T22:21:00Z | |
| dc.date.available | 2017-03-07T22:21:00Z | |
| dc.date.issued | 2011 | |
| dc.department | Biochemistry | en_US |
| dc.description.abstract | Protease enzyme catalyzes the hydrolysis of protein. Among the various proteases, bacterial proteases are the most significant when compared with animal and fungal proteases. The aim of the present study was to isolate the protease producing Bacillus strain a n f to optimize the media composition that supports the protease production. The enzyme from fish intestinal bacteria is an untouched bio-source for enzyme production. Hence, the isolation and enumeration of heterotrophic bacteria from gastrointestinal tracts of rohu, Labeo rohita was carried out. The isolates were selected based on the cellular moi-phology, growth conditions and biochemical tests. The best protease producing organism was screened and found as Bacillus subtilis by 16S rRNA sequencing. Then, production medium for Bacillus subtilis were optimized by using different pH, temperature, carbon and nitrogen sources for 48 hours fermentation period. The results obtained in the present study revealed that protease production can be optimized at pFI - 9.0, temperature 40°C by utilizing carbon source as glucose and nitrogen source as peptone. | en_US |
| dc.identifier.uri | https://ir.avinuty.ac.in/handle/avu/1939 | |
| dc.lang | English | en_US |
| dc.publisher.name | World Journal of Fish and Marine Sciences | en_US |
| dc.publisher.type | International | en_US |
| dc.title | Optimization of Protease Production by Bacillus subtilis Isolated from Mid Gut of Fresh Water Fish Labeo rohita | en_US |
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