A Study of Purification Profile and Molecular Weight of Fish Visceral Protease Precipitated With Different Precipitating Agents
| dc.category | Journal Article | |
| dc.contributor.author | Anitha Subash | |
| dc.date.accessioned | 2017-03-09T19:03:50Z | |
| dc.date.available | 2017-03-09T19:03:50Z | |
| dc.date.issued | 2014 | |
| dc.department | Biochemistry | en_US |
| dc.description.abstract | The study was aimed at managing the vast amounts of wastes of the redfish Lutjanus sebea to isolate an industrially useful enzyme-protease. The visceral organ waste.s of fish were collected, homogenized with Tris-HCI buffer and precipitated with varying concentrations of ammonium sulphate, aeetone and ethanol and was then purified by dialysis and Sephadex G-lOO column chromatography. The purification profile (the protein content, protease activity, specific activity, purification fold, and recovery %) were studied in the crude and partially purified samples. The results .showed that the activity was maximum when precipitated with 40-60% ammonium sulphate, 100% acetone and 40% ethanol. The fractions with the highest specific activity were selected for further purification by dialysis and Sephadex G-lOO column chromatography. The molecular weight of the isolated enzyme was determined by SDS-PAGE and Native PAGE and was to he found 27 kDa. The presence of protease was confirmed by zymography. | en_US |
| dc.identifier.uri | https://ir.avinuty.ac.in/handle/avu/2055 | |
| dc.lang | English | en_US |
| dc.publisher.name | International Journal of Current Research | en_US |
| dc.publisher.type | International | en_US |
| dc.title | A Study of Purification Profile and Molecular Weight of Fish Visceral Protease Precipitated With Different Precipitating Agents | en_US |
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