utilization Of Visceral Organ Waste Of Catia (Catia Catia) For The -Isolation, Purification And Partial Characterization Of Protease

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dc.categoryJournal Article
dc.contributor.authorAnitha Subash
dc.date.accessioned2017-03-07T21:36:16Z
dc.date.available2017-03-07T21:36:16Z
dc.date.issued2010
dc.departmentBiochemistryen_US
dc.description.abstractProtease is an industrially important enzyme isolated from various sources. Its isolation from the visceral organ waste of Catia, showed the highest specific activity in 50-60 % ammonium sulphate precipitation. The change in increase o f specific activity from 2.2 to 3.5 U/mg was noted with the dialyzed fraction. Further purification on Sephadex G-lOO column revealed an increase of specific activity from 3.5 to 3.7 U/mg. The molecular weight o f the enzyme was found to be 28.0 KDa. On Native PAGE it was found to be 48.0 KDa, which indicated the multimeric nature o f the isolated protein. The optimum pH and temperature o f the isolated enzyme was found to be 8 and 60°C respectively.en_US
dc.identifier.urihttps://ir.avinuty.ac.in/handle/avu/1924
dc.langEnglishen_US
dc.publisher.nameAdvanced Biotechnologyen_US
dc.publisher.typeNationalen_US
dc.titleutilization Of Visceral Organ Waste Of Catia (Catia Catia) For The -Isolation, Purification And Partial Characterization Of Proteaseen_US
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