Anitha Subash2017-03-072017-03-072011https://ir.avinuty.ac.in/handle/avu/1942Abstract: Rihhon ftsli (I'radiiptcrus trachyptcrus) i\ one of the mast commonly constimctlfisli in Coimbatore Ribbon fish possesses all the characteristics o f fish iivinp in very preat depth. Their fins especialiy and the membrane connectinp them are o f a very delicate and brittle structure. There is some potential fo r gaininp more value from fish waste. It is rich in valuable minerals, enzymes, pipments and favours that are required by many industries includinp food, apriculture, aquaculture and pharmaceuticals industries. Fish waste can also he utilised in the production o f orpanic fertilisers and composts, which have sipnificant benefits over chemical-based products. The aim of the present study was to utilize the visceral waste of ribbon fish (Trachipterus trachyptenis) for the extraction, purification and characterization o f protease. The wastes o f the fish Trachipterus trachyptenis were-coUected, cleaned, Itomopenizc’d, precipitated with ,4111111011111111 sulphate o f varyinp concentrations (0- S0%), purified by dialysis, Sephadex-G100 chromatapraphy, molecular weiphi determined by SD.S - PAGE, the presence o f protease confirmed by /ymopraphy and its activity assayed and atialyzed. The activity was found to be hiphest at the alkaline pH 7.H and maximuin protease activity remained at 50"C.